Normal human skin is superior to monkey oesophagus substrate for detection of circulating BP 180‐ NC 16A‐specific IgG antibodies in bullous pemphigoid
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چکیده
منابع مشابه
180 - kD Bullous Pemphigoid Antigen ( BP 180 ) Is Deficient in Generalized Atrophic Benign Epidermolysis Bullosa Marcel
Introduction Generalized atrophic benign epidermolysis bullosa (GABEB) is a form of nonlethal junctional epidermolysis bullosa characterized by universal alopecia and atrophy of the skin. We report a deficiency of the 180-kD bullous pemphigoid antigen in three patients with GABEB from unrelated families. We screened specimens of clinically normal skin from nine junctional epidermolysis bullosa ...
متن کاملAutoantibodies to bullous pemphigoid antigen 180 induce dermal-epidermal separation in cryosections of human skin.
Bullous pemphigoid is a subepidermal autoimmune blistering disease associated with autoantibodies to the hemidesmosomal bullous pemphigoid antigens 180 and 230. Most sera from bullous pemphigoid patients recognize epitopes within the N-terminal NC16A portion of the bullous pemphigoid 180 ectodomain. Using cryosections of human skin, patients' sera were shown to generate dermal-epidermal separat...
متن کاملHuman bullous pemphigoid antigen 2 transgenic skin elicits specific IgG in wild-type mice.
Bullous pemphigoid antigen 2 (BPAG2) is targeted by autoantibodies in patients with bullous pemphigoid (BP), and absent in patients with one type of epidermolysis bullosa (OMIM #226650). A keratin 14 promoter construct was used to produce transgenic (Tg) mice appropriately expressing human BPAG2 (hBPAG2) in murine epidermal basement membrane (BM). Grafts of Tg skin placed on gender-matched, syn...
متن کاملHuman amnion membrane as a substrate for the detection of autoantibodies in pemphigus vulgaris and bullous pemphigoid.
BACKGROUND Human amnion membrane (HAM) was suggested to be a superior antigenic substrate for immunoblotting in detecting autoantibodies of autoimmune bullous skin diseases. OBJECTIVES To determine the properties of HAM as an antigenic substrate for the detection of autoantibodies in pemphigus vulgaris and bullous pemphigoid. METHODS Immunomapping and tandem liquid chromatography mass spect...
متن کاملAnti-bullous pemphigoid 180 and 230 antibodies in a sample of unaffected subjects.
OBJECTIVE To evaluate the prevalence of autoantibodies against 2 hemidesmosomal proteins typically found in patients with bullous pemphigoid (BP), BP antigen II (BP180) and BP antigen I (BP230), in persons without BP. DESIGN Cross-sectional study. SETTING Academic medical center. Patients An age- and sex-stratified, random, population-based sample of local county patients seen during 2007: ...
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ژورنال
عنوان ژورنال: British Journal of Dermatology
سال: 2019
ISSN: 0007-0963,1365-2133
DOI: 10.1111/bjd.17313